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February 15, 2020
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November 29, 2019
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Abstract
Tumbuhan Asteraceae dilaporkan mengandung metabolit sekunder tinggi yang aktif sebagai antioksidan. Senyawa antioksidan juga digunakan untuk mencegah penuaan/reaksi glikasi. Penelitian ini bertujuan menentukan aktivitas antioksidan dan antiglikasi 7 tumbuhan Asteraceae. Daun Adenostema lavenia, Ageratum conyzoides, Dichrocephala integrifolia, Galinsoga parvifolia, Synedrella nodiflora, Mikania micrantha, dan Sphagneticola trilobata masing-masing diekstraksi menggunakan air. Ekstrak kemudian ditentukan kandungan total fenol, flavonoid, dan aktivitas antioksidan menggunakan metode 1,1-difenil-2-pikrilhidrazil (DPPH) dan 2,2’-azino-bis(3 ethylbenzothiazoline-6-sulfonic acid (ABTS), serta aktivitas antiglikasi. Ekstrak dengan kadar total fenolik dan flavonoid tertinggi adalah ekstrak M. micrantha. Ekstrak A. lavenia memiliki aktivitas antioksidan terhadap DPPH tertinggi dengan nilai IC50 sebesar 121,82 μg/mL dan A. conyzoides tertinggi terhadap ABTS dengan kapasitas sebesar 4,51 mg trolox ekivalen (TE)/g ekstrak. Aktivitas antiglikasi tertinggi pada ekstrak G. parvifolia sebesar 90,67% yang tidak berbeda nyata dengan ekstrak A. lavenia (87,87%) dan S. trilobata (87,38%). Secara keseluruhan ekstrak A. lavenia dan S. trilobata baik dikembangkan sebagai antioksidan dan antiglikasi.
Keywords
ABTS, Adenostema lavenia, Antiglikasi, Asteraceae, DPPH, Sphagneticola trilobata
Article Details
How to Cite
Budiarti, E., Batubara, I., & Ilmiawati, A. (2019). Potensi Beberapa Ekstrak Tumbuhan Asteraceae sebagai Antioksidan dan Antiglikasi. Jurnal Jamu Indonesia, 4(3), 103–111. https://doi.org/10.29244/jji.v4i3.161
References
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[AOAC]. The Association of Official Analytical Chemist. 2016. Official Methods of Analiysis Ed ke-20. Washington DC (US): Association of Official Analytical Chemist.
Azizah DN, Kumolowati E, Faramayuda F. 2014. Penetapan kadar flavonoid metode AlCl3 pada ekstrak metanol kulit buah kakao (Theobromo cacao L.) Kartika Jurnal Ilmiah Farmasi. 2(2): 45-49.
Batubara I, Mitsunaga T, Ohashi H. 2009. Screening antiacne potency of Indonesian medical plants: antibacterial, lipase inhibition, and antioxidant activities. J. Wood Sci. 55:230-235.
Bernatoniene J, Masteikova R, Davalgiene J, Peciura R, Gauryliene R, Bernatoniene R. 2011. Topical application of calendula officinalis (L);formulation and evaluation of hydrophilic with antioxidant activity. Journal of Medicinal Plant Research. 5:868-877.
Bhaigyabati T, Devi P, Bag G. C.2014. Total flavonoid content and antioxidant activity of aqueous rhizome extract of three Hedychium Species of Manipur Valley. Research Journal of Pharmaceutical Biological and Chemical Sciences. 5(5): 970-976.
Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 10:178-182.
Chen CM, Lin CY, Lin LC, Wan TC. 2012. Antioxidant activity and total phenolic contents of various Toona sinensis extracts. African Journal of Biotechnology. 11:13831-13837.
Dai J, Mumper RJ. 2010. Plant phenolic: extraction, analysis and their antioxidant and anticancer properties. Molecules. 15:7313-7352.
Dil FA, Ranjkesh Z, Goodarzi MT, 2019. A systematic review of antiglycation medicinal plants. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 13(2):1225-1229
Ditjen POM. 1995. Farmakope Indonesia. Edisi keempat. Jakarta (ID) : Departemen Kesehatan Republik Indonesia.
Erturk O, Demirbag Z. 2003. Scorzonare mollis Bieb (Compositae) bitkisinin antimikrobial aktivasi. Cerve Komura. 12:27-31.
Fauzan A, Praseptiangga D, Hartanto R, Pujiasmanto B. 2017. Characterization of the chemical composition of Adenostemma lavenia (L.) Kuntze and Adenostemma platyphyllum Cass. Earth and Environmental Sceince. 102(2018): 1-8.
Giancomoni PU, Rein G. 2001. Factors of skin aging share common mechanisms. Journal Biogerontology. 2:219-229.
Harvey D. 2009. Analytical Chemistry 2.0 Electronic versions. McGrawHill.
Heim KE, Tagliaferro AR, Bobilya DJ. 2002. Flavonoid antioxidants: chemistry,metabolism and structure-activity relationship. Journal ofNutritional Biochemistry. 13:572-584.
Idris A, Linatoc AC, Surayya MM, Aisha MA, Mohd FAB. 2018. Effect of light intensity of the total flavonoid and total phenolic contents of Mikaina mihranta and Tridax procumbens. 10(4):1-7.
Ko HH, Chang WL, Lu TM. 2008. Antityrosinase and antioxidant effect of ent-kaurane diterpenes from leaves of Broussonetia papyrifera. Journal Natural Product. 71: 1930-1933.
Kusumawati I, Djatmiko W, Rahman A, Studiawan H, Ekasari W. 2003. Eksplorasi keanekaragaman dan kandungan kimia tumbuhan obat di hutan tropis Gunung Arjuno. Jurnal Bahan Alam Indonesia. 2(3) : 100 – 104.
Lee SH, Sancheti SA, Bafna MR, Sancheti SS, Seo SY. 2011. Acetylcholineterase inhibitory and antioxidant properties of Rhododendron yedoense var.Poulhanense bark. Journal of Medicinal Plants Research. 5:248-254.
Ma BJ, Wen CN, Gao Y, Ren FC, Wang F, Liu JK. 2013. Ent-kaurane diterpenoids from the Wedelia trilobata. Natural Product and Bioprospect. 3: 107-111.
Marjoni MR, Nofita D, Rahmi N, Saifullah, Najia NA. 2018. Phenolic compound, flavonoids and antioxidant activity of Arum Manis leaves. International Journal of Green Pharmacy.12(3): 1-6.
Molyneux, P. 2004. The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Journal of Science and Technology. 26 (2): 211-219.
Ndlovu G, Gerda F, Maleva T, Werner C, Vanessa S. 2013. In vitro determinationof the anti-aging potential of four southern african medicinal plants. BMC Complementary and Alternative Medicine. 13:1-7.
Popova M, Bankova V, Butovska D, Petkov V. 2004. Validated methods for the quantification of biologically active constituents of poplar-type propolis. Phytochemical Analysis. 15: 235–240.
Povichit GV, Phrutivorapongkul A, Suttaji M, Chaiyasut C, Leelapornpisid P. 2010. Phenolic content and in vitro inhibitory effects on oxidation and protein glycation of some Thai medicinal plants. Pak. J. Pharm. Sci. 2(4): 403-408.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Evans CR. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26:1231-1237.
Salamah N, Farahan L. 2014. Uji aktivitas antioksidan ekstrak etanol herba pegagan (Centella asiatica (L.) Urb) dengan metode fosfomolibdat. Pharmaciana. 4:23-30.
Setiawan B, Suhartono E. 2005. Stress oksidatif dan peran antioksidan pada diabetes melitus. Majalah kedokteran Indonesia. 55(2):87-90.
Shaich KM, Tian X, Xie J. 2015. Reprint of hurdles and pitfalls in measuring antioxidant efficacy a critical evaluation of ABTS, DPPH, and ORAC assays. Journal of Functional Foods. 1-15.
Shalaby EA, Shanab SMM. 2013. Review: Antioxidant compounds, assays of determination and mode of action. African Journal Pharmacy andPharmacology. 7:528-539.
Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenol and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology. 299C: 152-178.
Stallings AF, Lupo MP. 2009. Practical uses of botanicals in skin care. Journal of Clinical Aesthetic Dermatology. 2:36-40.
Wang CC, Chu CY, Chu KO, Choy KW, Khaw KS, Rogers MS, Pang CP. 2004. Trolox-equivalent antioxidant capacity assay versus oxygen radical absorbance capacity assay in plasma. Clinical Chemistry. 50:952-954.
Wegiera M, Smolarz HD, Jedruch M, Korczat M, Kopron K. 2012. Cytotoxic effect of some medicinal plant from Asteraceae family on J-45.01 leukemic cell line—pilot study. Acta Policy Pharmacology. 69(2): 1-8.
Yagi S, Drouart N, Bourgaud F, Henry M, Chapleur Y, Mattar DL. 2013. Antioxidant and antiglycation properties of Hydnora johannis roots. South African Journal of Botany. 84:124-127.
Zahra U, Kartika Y, Batubara I, Darusman LK, Maddu A. 2016. Short communication: Screening the potency of Zingiberaceae leaves as antioxidant and antiaging agent. Nusantara Bioscience. 8:221-225.
References
[AOAC]. The Association of Official Analytical Chemist. 2016. Official Methods of Analiysis Ed ke-20. Washington DC (US): Association of Official Analytical Chemist.
Azizah DN, Kumolowati E, Faramayuda F. 2014. Penetapan kadar flavonoid metode AlCl3 pada ekstrak metanol kulit buah kakao (Theobromo cacao L.) Kartika Jurnal Ilmiah Farmasi. 2(2): 45-49.
Batubara I, Mitsunaga T, Ohashi H. 2009. Screening antiacne potency of Indonesian medical plants: antibacterial, lipase inhibition, and antioxidant activities. J. Wood Sci. 55:230-235.
Bernatoniene J, Masteikova R, Davalgiene J, Peciura R, Gauryliene R, Bernatoniene R. 2011. Topical application of calendula officinalis (L);formulation and evaluation of hydrophilic with antioxidant activity. Journal of Medicinal Plant Research. 5:868-877.
Bhaigyabati T, Devi P, Bag G. C.2014. Total flavonoid content and antioxidant activity of aqueous rhizome extract of three Hedychium Species of Manipur Valley. Research Journal of Pharmaceutical Biological and Chemical Sciences. 5(5): 970-976.
Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 10:178-182.
Chen CM, Lin CY, Lin LC, Wan TC. 2012. Antioxidant activity and total phenolic contents of various Toona sinensis extracts. African Journal of Biotechnology. 11:13831-13837.
Dai J, Mumper RJ. 2010. Plant phenolic: extraction, analysis and their antioxidant and anticancer properties. Molecules. 15:7313-7352.
Dil FA, Ranjkesh Z, Goodarzi MT, 2019. A systematic review of antiglycation medicinal plants. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 13(2):1225-1229
Ditjen POM. 1995. Farmakope Indonesia. Edisi keempat. Jakarta (ID) : Departemen Kesehatan Republik Indonesia.
Erturk O, Demirbag Z. 2003. Scorzonare mollis Bieb (Compositae) bitkisinin antimikrobial aktivasi. Cerve Komura. 12:27-31.
Fauzan A, Praseptiangga D, Hartanto R, Pujiasmanto B. 2017. Characterization of the chemical composition of Adenostemma lavenia (L.) Kuntze and Adenostemma platyphyllum Cass. Earth and Environmental Sceince. 102(2018): 1-8.
Giancomoni PU, Rein G. 2001. Factors of skin aging share common mechanisms. Journal Biogerontology. 2:219-229.
Harvey D. 2009. Analytical Chemistry 2.0 Electronic versions. McGrawHill.
Heim KE, Tagliaferro AR, Bobilya DJ. 2002. Flavonoid antioxidants: chemistry,metabolism and structure-activity relationship. Journal ofNutritional Biochemistry. 13:572-584.
Idris A, Linatoc AC, Surayya MM, Aisha MA, Mohd FAB. 2018. Effect of light intensity of the total flavonoid and total phenolic contents of Mikaina mihranta and Tridax procumbens. 10(4):1-7.
Ko HH, Chang WL, Lu TM. 2008. Antityrosinase and antioxidant effect of ent-kaurane diterpenes from leaves of Broussonetia papyrifera. Journal Natural Product. 71: 1930-1933.
Kusumawati I, Djatmiko W, Rahman A, Studiawan H, Ekasari W. 2003. Eksplorasi keanekaragaman dan kandungan kimia tumbuhan obat di hutan tropis Gunung Arjuno. Jurnal Bahan Alam Indonesia. 2(3) : 100 – 104.
Lee SH, Sancheti SA, Bafna MR, Sancheti SS, Seo SY. 2011. Acetylcholineterase inhibitory and antioxidant properties of Rhododendron yedoense var.Poulhanense bark. Journal of Medicinal Plants Research. 5:248-254.
Ma BJ, Wen CN, Gao Y, Ren FC, Wang F, Liu JK. 2013. Ent-kaurane diterpenoids from the Wedelia trilobata. Natural Product and Bioprospect. 3: 107-111.
Marjoni MR, Nofita D, Rahmi N, Saifullah, Najia NA. 2018. Phenolic compound, flavonoids and antioxidant activity of Arum Manis leaves. International Journal of Green Pharmacy.12(3): 1-6.
Molyneux, P. 2004. The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Journal of Science and Technology. 26 (2): 211-219.
Ndlovu G, Gerda F, Maleva T, Werner C, Vanessa S. 2013. In vitro determinationof the anti-aging potential of four southern african medicinal plants. BMC Complementary and Alternative Medicine. 13:1-7.
Popova M, Bankova V, Butovska D, Petkov V. 2004. Validated methods for the quantification of biologically active constituents of poplar-type propolis. Phytochemical Analysis. 15: 235–240.
Povichit GV, Phrutivorapongkul A, Suttaji M, Chaiyasut C, Leelapornpisid P. 2010. Phenolic content and in vitro inhibitory effects on oxidation and protein glycation of some Thai medicinal plants. Pak. J. Pharm. Sci. 2(4): 403-408.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Evans CR. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26:1231-1237.
Salamah N, Farahan L. 2014. Uji aktivitas antioksidan ekstrak etanol herba pegagan (Centella asiatica (L.) Urb) dengan metode fosfomolibdat. Pharmaciana. 4:23-30.
Setiawan B, Suhartono E. 2005. Stress oksidatif dan peran antioksidan pada diabetes melitus. Majalah kedokteran Indonesia. 55(2):87-90.
Shaich KM, Tian X, Xie J. 2015. Reprint of hurdles and pitfalls in measuring antioxidant efficacy a critical evaluation of ABTS, DPPH, and ORAC assays. Journal of Functional Foods. 1-15.
Shalaby EA, Shanab SMM. 2013. Review: Antioxidant compounds, assays of determination and mode of action. African Journal Pharmacy andPharmacology. 7:528-539.
Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenol and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology. 299C: 152-178.
Stallings AF, Lupo MP. 2009. Practical uses of botanicals in skin care. Journal of Clinical Aesthetic Dermatology. 2:36-40.
Wang CC, Chu CY, Chu KO, Choy KW, Khaw KS, Rogers MS, Pang CP. 2004. Trolox-equivalent antioxidant capacity assay versus oxygen radical absorbance capacity assay in plasma. Clinical Chemistry. 50:952-954.
Wegiera M, Smolarz HD, Jedruch M, Korczat M, Kopron K. 2012. Cytotoxic effect of some medicinal plant from Asteraceae family on J-45.01 leukemic cell line—pilot study. Acta Policy Pharmacology. 69(2): 1-8.
Yagi S, Drouart N, Bourgaud F, Henry M, Chapleur Y, Mattar DL. 2013. Antioxidant and antiglycation properties of Hydnora johannis roots. South African Journal of Botany. 84:124-127.
Zahra U, Kartika Y, Batubara I, Darusman LK, Maddu A. 2016. Short communication: Screening the potency of Zingiberaceae leaves as antioxidant and antiaging agent. Nusantara Bioscience. 8:221-225.
Azizah DN, Kumolowati E, Faramayuda F. 2014. Penetapan kadar flavonoid metode AlCl3 pada ekstrak metanol kulit buah kakao (Theobromo cacao L.) Kartika Jurnal Ilmiah Farmasi. 2(2): 45-49.
Batubara I, Mitsunaga T, Ohashi H. 2009. Screening antiacne potency of Indonesian medical plants: antibacterial, lipase inhibition, and antioxidant activities. J. Wood Sci. 55:230-235.
Bernatoniene J, Masteikova R, Davalgiene J, Peciura R, Gauryliene R, Bernatoniene R. 2011. Topical application of calendula officinalis (L);formulation and evaluation of hydrophilic with antioxidant activity. Journal of Medicinal Plant Research. 5:868-877.
Bhaigyabati T, Devi P, Bag G. C.2014. Total flavonoid content and antioxidant activity of aqueous rhizome extract of three Hedychium Species of Manipur Valley. Research Journal of Pharmaceutical Biological and Chemical Sciences. 5(5): 970-976.
Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 10:178-182.
Chen CM, Lin CY, Lin LC, Wan TC. 2012. Antioxidant activity and total phenolic contents of various Toona sinensis extracts. African Journal of Biotechnology. 11:13831-13837.
Dai J, Mumper RJ. 2010. Plant phenolic: extraction, analysis and their antioxidant and anticancer properties. Molecules. 15:7313-7352.
Dil FA, Ranjkesh Z, Goodarzi MT, 2019. A systematic review of antiglycation medicinal plants. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 13(2):1225-1229
Ditjen POM. 1995. Farmakope Indonesia. Edisi keempat. Jakarta (ID) : Departemen Kesehatan Republik Indonesia.
Erturk O, Demirbag Z. 2003. Scorzonare mollis Bieb (Compositae) bitkisinin antimikrobial aktivasi. Cerve Komura. 12:27-31.
Fauzan A, Praseptiangga D, Hartanto R, Pujiasmanto B. 2017. Characterization of the chemical composition of Adenostemma lavenia (L.) Kuntze and Adenostemma platyphyllum Cass. Earth and Environmental Sceince. 102(2018): 1-8.
Giancomoni PU, Rein G. 2001. Factors of skin aging share common mechanisms. Journal Biogerontology. 2:219-229.
Harvey D. 2009. Analytical Chemistry 2.0 Electronic versions. McGrawHill.
Heim KE, Tagliaferro AR, Bobilya DJ. 2002. Flavonoid antioxidants: chemistry,metabolism and structure-activity relationship. Journal ofNutritional Biochemistry. 13:572-584.
Idris A, Linatoc AC, Surayya MM, Aisha MA, Mohd FAB. 2018. Effect of light intensity of the total flavonoid and total phenolic contents of Mikaina mihranta and Tridax procumbens. 10(4):1-7.
Ko HH, Chang WL, Lu TM. 2008. Antityrosinase and antioxidant effect of ent-kaurane diterpenes from leaves of Broussonetia papyrifera. Journal Natural Product. 71: 1930-1933.
Kusumawati I, Djatmiko W, Rahman A, Studiawan H, Ekasari W. 2003. Eksplorasi keanekaragaman dan kandungan kimia tumbuhan obat di hutan tropis Gunung Arjuno. Jurnal Bahan Alam Indonesia. 2(3) : 100 – 104.
Lee SH, Sancheti SA, Bafna MR, Sancheti SS, Seo SY. 2011. Acetylcholineterase inhibitory and antioxidant properties of Rhododendron yedoense var.Poulhanense bark. Journal of Medicinal Plants Research. 5:248-254.
Ma BJ, Wen CN, Gao Y, Ren FC, Wang F, Liu JK. 2013. Ent-kaurane diterpenoids from the Wedelia trilobata. Natural Product and Bioprospect. 3: 107-111.
Marjoni MR, Nofita D, Rahmi N, Saifullah, Najia NA. 2018. Phenolic compound, flavonoids and antioxidant activity of Arum Manis leaves. International Journal of Green Pharmacy.12(3): 1-6.
Molyneux, P. 2004. The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Journal of Science and Technology. 26 (2): 211-219.
Ndlovu G, Gerda F, Maleva T, Werner C, Vanessa S. 2013. In vitro determinationof the anti-aging potential of four southern african medicinal plants. BMC Complementary and Alternative Medicine. 13:1-7.
Popova M, Bankova V, Butovska D, Petkov V. 2004. Validated methods for the quantification of biologically active constituents of poplar-type propolis. Phytochemical Analysis. 15: 235–240.
Povichit GV, Phrutivorapongkul A, Suttaji M, Chaiyasut C, Leelapornpisid P. 2010. Phenolic content and in vitro inhibitory effects on oxidation and protein glycation of some Thai medicinal plants. Pak. J. Pharm. Sci. 2(4): 403-408.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Evans CR. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26:1231-1237.
Salamah N, Farahan L. 2014. Uji aktivitas antioksidan ekstrak etanol herba pegagan (Centella asiatica (L.) Urb) dengan metode fosfomolibdat. Pharmaciana. 4:23-30.
Setiawan B, Suhartono E. 2005. Stress oksidatif dan peran antioksidan pada diabetes melitus. Majalah kedokteran Indonesia. 55(2):87-90.
Shaich KM, Tian X, Xie J. 2015. Reprint of hurdles and pitfalls in measuring antioxidant efficacy a critical evaluation of ABTS, DPPH, and ORAC assays. Journal of Functional Foods. 1-15.
Shalaby EA, Shanab SMM. 2013. Review: Antioxidant compounds, assays of determination and mode of action. African Journal Pharmacy andPharmacology. 7:528-539.
Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenol and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology. 299C: 152-178.
Stallings AF, Lupo MP. 2009. Practical uses of botanicals in skin care. Journal of Clinical Aesthetic Dermatology. 2:36-40.
Wang CC, Chu CY, Chu KO, Choy KW, Khaw KS, Rogers MS, Pang CP. 2004. Trolox-equivalent antioxidant capacity assay versus oxygen radical absorbance capacity assay in plasma. Clinical Chemistry. 50:952-954.
Wegiera M, Smolarz HD, Jedruch M, Korczat M, Kopron K. 2012. Cytotoxic effect of some medicinal plant from Asteraceae family on J-45.01 leukemic cell line—pilot study. Acta Policy Pharmacology. 69(2): 1-8.
Yagi S, Drouart N, Bourgaud F, Henry M, Chapleur Y, Mattar DL. 2013. Antioxidant and antiglycation properties of Hydnora johannis roots. South African Journal of Botany. 84:124-127.
Zahra U, Kartika Y, Batubara I, Darusman LK, Maddu A. 2016. Short communication: Screening the potency of Zingiberaceae leaves as antioxidant and antiaging agent. Nusantara Bioscience. 8:221-225.