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Aging is a natural process that occurs in every living organism, including humans. Skin wrinkle, caused by deterioration activity of the elastase enzyme, is one example of the aging process. The activity of the elastase enzyme could be inhibited by flavonoid and phenolic compounds. Zodia (Evodia suaveolens) from the family of Rutaceae is one of the indigenous Indonesian plants widely known as rich in flavonoid and phenolic compounds. The purpose of this study is to explore antioxidant and anti-aging activity from ethanolic extract of fruit and leaf Zodia. The extracts were prepared by refluxing fruit and leaf zodia in 96% ethanol. The obtained extracts were characterized for the phytochemical constituent and its antioxidant activity determined using the 2,2-diphenyl-1-picrylhydrazyl method as well as anti-elastase activity for anti-aging evaluation. Phytochemical analysis of both samples showed the presence of flavonoids, saponins, tannins, steroids and essential oils. The calculation of IC50 in antioxidant activity using the DPPH method showed that ethanolic extract of zodia leaf (264.15 ppm) was higher compared to fruit extract (121.59 ppm). The fruit extract was further tested for anti elastase activity with IC50 of 145.67 ppm. In conclusion, the presence of phytochemicals in zodia fruit may be responsible for the antioxidant and anti-aging activities. 


antioxidants anti elastases Evodia suaveolens

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How to Cite
Greesty F. Swandiny, Yesi Desmiaty, & Haura Usna Rahmah. (2022). Antioxidant and Anti-aging Activities from Zodia (Evodia suaveolens J.R. Forst & G. Forst) Fruit and Leaf Extract. Jurnal Jamu Indonesia, 7(1), 20–24.


  1. Artanti N, Maryani F, Mulyani H, Dewi RT, Saraswati V, Murniasih T. 2016. Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges. Annales Bogoriensis. 20(1): 23-28
  2. Harborne JB. 1987. Metode fitokimia penuntun cara modern menganalisis tumbuhan. Edisi II. Diterjemahkan oleh Padmawinata K., Soediro I. Bandung: ITB; 1987.
  3. Harman D. 1956. Aging: A Theory Based on Free Radical and Radiation Chemistry. J Gerontol. 11: 298-300.
  4. Kardinan A. 2004. Zodia (Evodia suaveolens) Tanaman Pengusir Nyamuk. Tabloid Sinar Tani, 23 Juni 2004.
  5. Kirkwood TB and Kowald A. 2012. The Free-Radical Theory of Aging - Older, Wiser and Still Alive: Modelling Positional Effects of The Primary Targets of ROS Reveals New Support. Bioassays. 34: 692-700.
  6. Korac RR, Khambholja KM. 2011. Potential of Herb in Skin Protection from Ultraviolet Radiation. Pharmacognosy Reviews. 5(10): 164-173.
  7. Lestari MS, Himawan T, Abadi AL, Retnowati R. 2015. Toxicity and phytochemistry test of methanol extract of several plants from papua using Brine Shrimp Lethality Test (BSLT). JOCPR. 7(4): 866-871
  8. Moeloek FA. 2006. Herbal Traditional Medicine: National perspective and policies in Indonesia. Jurnal Bahan Alam Indonesia. 5(1): 293-297.
  9. Molyneux P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 26(2): 211-219
  10. Moon JY, Yim EY, Song G, 2010. Screening of elastase and tyrosinase inhibitory activity from Jeju Island plants. EurAsia J BioSci. 4: 41-45.
  11. Mutakin, Yunita W and Nikodemus TW. 2021. Isolation and Characterization of Chemical Compounds From n-Hexane Extract of Zodia (Evodia suaveolens Scheff.) Leaves. Indonesian Journal of Pharmaceutical Science and Technology. 8(2): 86-95.
  12. Panche AN, Diwan AD and Chandra SR. 2016. Flavonoids: An Overview. Journal of Nutritional Sciences. 5(47): 1-15.
  13. Pietta Pier-Georgio. 2000. Flavonoids as Antioxidant. Journal of Natural Products. 63: 1035-1042.
  14. Pribadi ER. 2009. Pasokan permintaan tanaman obat Indonesia serta arah penelitiannya dan perkembangannya. Perspektif. 8(1): 52-54.
  15. Stadtman ER. 1992. Protein oxidation and aging. Science. 257: 1220–1224.
  16. Shukla V, Mishra SK and Pant HC. 2011. Oxidative Stress in Neurodegeneration. Advances in Pharmacological Sciences. 2011: 1-15
  17. Thring SA, Hill P, and Naughton PD. 2009. Anti-collagenase, anti-elastase, and anti- oxidant activities of extracts from 21 plants. BMC Complementary and Alternative Medicine. 9(27): 1-10.
  18. Trachootham D, Alexandre J and Huang P. 2009. Targeting Cancer Cells by ROS-mediated Mechanisma: A Radical Terapeutic Approach? Nature Review Drug Discovery. 8: 579-591
  19. Weihermann AC, Lorencini M, Brohem CA and de Carvalho M. 2017. Elastin Structure and Its Involvement in Photoageing. International Journal of Cosmetic Science. 39: 241-247