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Submitted
July 11, 2020
Accepted
December 28, 2021
Published
July 5, 2021
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Abstract
Sauropus androgynus (L.) Merr (Phyllanthaceae), also known as katuk in Indonesia, is a plant that grows in tropical climates. The study aimed to determine the total flavonoid levels of hydroethanolic extract of katuk leaves from three regions with different altitudes. Dried katuk leaves were macerated using hydroethanolic (the mixture of ethanol:H2O (7:3, v/v)) as solvent extraction at room temperature. Determination of flavonoid levels was carried out by the colorimetry method using AlCl3 reagent. Quercetin was used as a reference. A UV-Visible spectrophotometer measured the absorbance at the maximum wavelength of 434.50 nm. The total flavonoids level of hydroethanolic extract of katuk leaves from a different region with low-altitude (Bogor), medium-altitude (Sleman), and high-altitude (Bandung) are 8.56±0.63 mgQE/g, 4.67±0.30 mgQE/g, and 9.72±0.24 mgQE/g, respectively. Thus, it can be concluded that there are differences in total flavonoid levels of hydroethanolic extract of katuk leaves from three regions with different altitudes.
Keywords
Flavonoid
Katuk
Ketinggian
Sauropus androgynus (L.) Merr
Altitude
Article Details
How to Cite
Ni Putu Ermi Hikmawanti, Hayati, & Yeni Andriyani. (2021). Total Flavonoid Content in Hydro-ethanolic Extract of Sauropus androgynus (L.) Merr Leaves from Three Regions with Different Altitude. Jurnal Jamu Indonesia, 6(2), 61–67. https://doi.org/10.29244/jji.v6i2.195
References
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Agrawal, S. K. et al. (2014) ‘Multivitamin plant : pharmacognostical standardization and phytochemical profile of its leaves’, Journal of Pharmacy Research, 8(7), pp. 920–925.
Cechibnel-Filho, V. (2012) Plant Bioactives and Drug Discovery: Principle, Practice, adn Perspectives. New Jersey.
Chang, C. et al. (2002) ‘Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods’, Journal of Food and Drug Analysis, 10(3), pp. 178–182.
Chang, C. C. et al. (2002) ‘Estimation of total flavonoid content in propolis by two complementary colometric methods’, Journal of Food and Drug Analysis, 10(3), pp. 178–182.
Departemen Kesehatan RI (2000) ‘Parameter Standar Umum Ekstrak Tumbuhan’. Jakarta: Ministry of Health, Indonesia, p. 76.
Departemen Kesehatan RI (2010) Suplemen Farmakope Herbal Indonesia. Jakarta: Direktorat Jenderal POM, Departemen Kesehatan Republik Indonesia.
Direktorat Obat Asli Indonesia (2008) Taksonomi: Koleksi Tanaman Obat Kebun Tanaman Obat Citeureup. Jakarta: Badan POM RI.
Djamil, R. and Zaidan, S. (2017) ‘Isolasi Senyawa Flavonoid dari Ekstrak Metanol Daun Katuk (Sauropus androgynus (L.) Merr), Euphorbiaceae’, Jurnal Ilmu Kefarmasian Indonesia, 14(1), pp. 57–61.
Hanani, E. (2015) Analisis Fitokimia (In Bahasa). Jakarta: Buku Kedokteran EGC.
Ministry of Health Republic of Indonesia (2008) Farmakope Herbal Indonesia (Indonesian Herb Pharmacopoeia). 1st Editio. Jakarta: Ministry of Health Republic of Indonesia.
Petrus, A. J. A. (2013) ‘Sauropus androgynus (L.) merrill-a potentially nutritive functional leafy-vegetable’, Asian Journal of Chemistry, 25(17), pp. 9425–9433.
Tiwari, P. . et al. (2011) ‘Phytochemical screening and Extraction: A Review’, Internationale Pharmaceutica Sciencia, 1(1), pp. 98–106.
World Health Organization (1998) Quality control methods for medicinal plant materials. Geneva, Switzerland: World Health Organization.
References
Agrawal, S. K. et al. (2014) ‘Multivitamin plant : pharmacognostical standardization and phytochemical profile of its leaves’, Journal of Pharmacy Research, 8(7), pp. 920–925.
Cechibnel-Filho, V. (2012) Plant Bioactives and Drug Discovery: Principle, Practice, adn Perspectives. New Jersey.
Chang, C. et al. (2002) ‘Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods’, Journal of Food and Drug Analysis, 10(3), pp. 178–182.
Chang, C. C. et al. (2002) ‘Estimation of total flavonoid content in propolis by two complementary colometric methods’, Journal of Food and Drug Analysis, 10(3), pp. 178–182.
Departemen Kesehatan RI (2000) ‘Parameter Standar Umum Ekstrak Tumbuhan’. Jakarta: Ministry of Health, Indonesia, p. 76.
Departemen Kesehatan RI (2010) Suplemen Farmakope Herbal Indonesia. Jakarta: Direktorat Jenderal POM, Departemen Kesehatan Republik Indonesia.
Direktorat Obat Asli Indonesia (2008) Taksonomi: Koleksi Tanaman Obat Kebun Tanaman Obat Citeureup. Jakarta: Badan POM RI.
Djamil, R. and Zaidan, S. (2017) ‘Isolasi Senyawa Flavonoid dari Ekstrak Metanol Daun Katuk (Sauropus androgynus (L.) Merr), Euphorbiaceae’, Jurnal Ilmu Kefarmasian Indonesia, 14(1), pp. 57–61.
Hanani, E. (2015) Analisis Fitokimia (In Bahasa). Jakarta: Buku Kedokteran EGC.
Ministry of Health Republic of Indonesia (2008) Farmakope Herbal Indonesia (Indonesian Herb Pharmacopoeia). 1st Editio. Jakarta: Ministry of Health Republic of Indonesia.
Petrus, A. J. A. (2013) ‘Sauropus androgynus (L.) merrill-a potentially nutritive functional leafy-vegetable’, Asian Journal of Chemistry, 25(17), pp. 9425–9433.
Tiwari, P. . et al. (2011) ‘Phytochemical screening and Extraction: A Review’, Internationale Pharmaceutica Sciencia, 1(1), pp. 98–106.
World Health Organization (1998) Quality control methods for medicinal plant materials. Geneva, Switzerland: World Health Organization.
Cechibnel-Filho, V. (2012) Plant Bioactives and Drug Discovery: Principle, Practice, adn Perspectives. New Jersey.
Chang, C. et al. (2002) ‘Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods’, Journal of Food and Drug Analysis, 10(3), pp. 178–182.
Chang, C. C. et al. (2002) ‘Estimation of total flavonoid content in propolis by two complementary colometric methods’, Journal of Food and Drug Analysis, 10(3), pp. 178–182.
Departemen Kesehatan RI (2000) ‘Parameter Standar Umum Ekstrak Tumbuhan’. Jakarta: Ministry of Health, Indonesia, p. 76.
Departemen Kesehatan RI (2010) Suplemen Farmakope Herbal Indonesia. Jakarta: Direktorat Jenderal POM, Departemen Kesehatan Republik Indonesia.
Direktorat Obat Asli Indonesia (2008) Taksonomi: Koleksi Tanaman Obat Kebun Tanaman Obat Citeureup. Jakarta: Badan POM RI.
Djamil, R. and Zaidan, S. (2017) ‘Isolasi Senyawa Flavonoid dari Ekstrak Metanol Daun Katuk (Sauropus androgynus (L.) Merr), Euphorbiaceae’, Jurnal Ilmu Kefarmasian Indonesia, 14(1), pp. 57–61.
Hanani, E. (2015) Analisis Fitokimia (In Bahasa). Jakarta: Buku Kedokteran EGC.
Ministry of Health Republic of Indonesia (2008) Farmakope Herbal Indonesia (Indonesian Herb Pharmacopoeia). 1st Editio. Jakarta: Ministry of Health Republic of Indonesia.
Petrus, A. J. A. (2013) ‘Sauropus androgynus (L.) merrill-a potentially nutritive functional leafy-vegetable’, Asian Journal of Chemistry, 25(17), pp. 9425–9433.
Tiwari, P. . et al. (2011) ‘Phytochemical screening and Extraction: A Review’, Internationale Pharmaceutica Sciencia, 1(1), pp. 98–106.
World Health Organization (1998) Quality control methods for medicinal plant materials. Geneva, Switzerland: World Health Organization.