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February 21, 2019
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November 30, 2018
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Abstract
Spektrofotometer Quali-Vis digunakan untuk mengklasifikasikan 3 sampel daun wungu dengan usia panen berbeda. Penelitian ini bertujuan mengevaluasi model klasifikasi menggunakan spektrofotometer Quali-Vis secara kualitatif. Model dikembangkan dengan akuisisi data menggunakan principle component analysis (PCA) dan partial least square-discriminant analysis (PLS-DA). Karakteristik awal sampel daun wungu ditentukan dengan analisis sidik jari kromatografi lapis tipis (KLT) yang menampilkan profil yang berbeda pada masing-masing usia panen. Perbedaan ini menjadi dasar klasifikasi untuk model PLS-DA yang terdiri atas model usia 1 bulan, 2 bulan, dan 3 bulan, yang menghasilkan rerata R2pred = 0.7794 dan root mean square error predict (RMSEP) = 0.2180. Model divalidasi dengan menentukan nilai parameter sensitivitas, spesifitas, presisi, dan akurasi yang menghasilkan nilai rerata masing-masing 0.95, 0.99, 0.98, dan 0.98 kesesuaian prediksi. Nilai-nilai ini menunjukkan bahwa model yang dikembangkan memiliki kemampuan klasifikasi yang cukup baik.
Keywords
Daun wungu (Graptophyllum pictum)
PCA
PLS-DA
Quali-vis
Sidikjari KLT
Article Details
How to Cite
Putri, S. A., Heryanto, R., & Rohaeti, E. (2018). Spektrofotometer Quali-Vis dan Kemometrika untuk Klasifikasi Kualitas Daun Wungu (Graptophyllum pictum). Jurnal Jamu Indonesia, 3(3), 89–101. https://doi.org/10.29244/jji.v3i3.66
References
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[Depkes RI] Departemen Kesehatan Republik Indonesia. 2010. Farmakope Herbal Indonesia Edisi I. Jakarta (ID): Depkes RI.
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Anuradha VE, Jaleel CA, Salem MA, Gomathinayagam M, Panneerselvam R. 2010. Plant growth regulators induced changes in antioksidant potential and andrographolide content in Andrographis paniculata Wall ex Nees. Pestic Biochem Physiol. 98:312-316.
Aminah, Muflihunna A, Abidin Z. 2016. Uji aktivitas antioksidan fraksi etil asetat daun wungu (Graptophyllum pictum (Linn) Griff) dengan metode FRAP (Ferric Reducing Antioxidant Power). As-syifaa. 8(1):39-44
Astuti AI. 2015. Kuantifikasi kurkuminoid dalam temulawak menggunakan spektrofotometer reflektans berbasis CCD. [skripsi]. Bogor (ID): Institut Pertanian Bogor.
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Cuadros-Rodriguez L, Perez-Castano E, Ruiz-SamblasC. 2016. Quality performance metrics in multivariate classification methods for qualitative analysis. TRAC-Trend Anal Chem. 80:612-624
Dalimartha. 1999. Ramuan Tradisional Untuk Pengobatan Hepatitis. Jakarta: Penebar Swadaya.
Gromski P, Muhammadali H, Ellis D, Xu Y, Correa E. 2015. A tutorial review: metabolomics and partial least squares-discriminant analysis-a marriage of convenience or a shotgun wedding. Anal Chim Acta. 879:10-23
Hafsah N. 2017. Validasi kualitatif model klasifikasi kualitas temu lawak menggunakan spektrofotometer reflektans Quali-Vis dan Kemometrika[skripsi]. Bogor (ID): Institut Pertanian Bogor.
Jiangseubchatveera N, Liawruangrath S, Teerawutgulrag A, Santiarworn D, Pyne SG, Liawruangrath B. 2017. Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff. Chiang Mai J SCI. 44:193-202.
Mardiana E. 2017. Uji kinerja analitik spektrofotometer sinar tampak berbasis reflektans menggunakan pewarna sudan. [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Morris AS. 2001. Measurement and Instrumentation Principles. London (GB):
Butterworth-Heinemann.
Rahma N. 2016. Spektrofotometer reflektans sinar tampak dan kemometrika sebagai kendali mutu daun jati belanda (Guazuma ulmifokia). [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Sanda Fm, Victor Me, Monica Ta, Alina C. 2012. Spectrophotometric Measurements Techniques for Fermentation Process. Romania: University Of Oradea
Singh SK, Jha SK, Chaudhary, Yadava RDS, Rai SB. 2010. Quality control of herbal medicines by using spectroscopic techniques and multivariate statistical analysis. Phram Biol. 48:134-141
Stoenoiu CM, Bolbocoa AD, Jansthi L. 2006. Mobile phase optimization for steroid separation. Med Informatics. 16:141-148
Wakabayashi F, Hamada K. 2006. A DVD spectroscope: a simple, high-resolution classroom spectrosope. J Chem Educ. 83:56-58
Wiart C. 2006. Medicinal Plants of the Asia-Pacific: Drugs for the Future. Singapura: World Scientific Publishingh Co, Ltd.
Zeaiter M, Rutledge DN. 2009. Comprehensive Chemometrics. Amsterdam (NL): Elsevier.
References
[AOAC] Association of Official Analytical Chemist. 2007. Official method of Analysis of Analysis. Ed ke-14. Aelington: AOAC.
CAMO. 2006. The Unscrambler User Manual: The Unscrambler Methods. New
York (US): CAMO Software AS.
[Depkes RI] Departemen Kesehatan Republik Indonesia. 2010. Farmakope Herbal Indonesia Edisi I. Jakarta (ID): Depkes RI.
Antari PD. 2015. Spektrofotometer Uv-Vis reflektans dan kemometrika sebagai teknik klasifikasi spesimen jaringan kanker serviks dan normal. [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Anuradha VE, Jaleel CA, Salem MA, Gomathinayagam M, Panneerselvam R. 2010. Plant growth regulators induced changes in antioksidant potential and andrographolide content in Andrographis paniculata Wall ex Nees. Pestic Biochem Physiol. 98:312-316.
Aminah, Muflihunna A, Abidin Z. 2016. Uji aktivitas antioksidan fraksi etil asetat daun wungu (Graptophyllum pictum (Linn) Griff) dengan metode FRAP (Ferric Reducing Antioxidant Power). As-syifaa. 8(1):39-44
Astuti AI. 2015. Kuantifikasi kurkuminoid dalam temulawak menggunakan spektrofotometer reflektans berbasis CCD. [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Blum-Silva CH, Chaves VC, Schenkel EP, Coelho GC, Reginatto FH. 2015. The influence of leaf age on methylxanthines, total phenolic content, and free radical scavenging capacity of Ilex paraguariensis aqueous extracts. Rev. Bras. Farmacogn. 25:1-6
Cuadros-Rodriguez L, Perez-Castano E, Ruiz-SamblasC. 2016. Quality performance metrics in multivariate classification methods for qualitative analysis. TRAC-Trend Anal Chem. 80:612-624
Dalimartha. 1999. Ramuan Tradisional Untuk Pengobatan Hepatitis. Jakarta: Penebar Swadaya.
Gromski P, Muhammadali H, Ellis D, Xu Y, Correa E. 2015. A tutorial review: metabolomics and partial least squares-discriminant analysis-a marriage of convenience or a shotgun wedding. Anal Chim Acta. 879:10-23
Hafsah N. 2017. Validasi kualitatif model klasifikasi kualitas temu lawak menggunakan spektrofotometer reflektans Quali-Vis dan Kemometrika[skripsi]. Bogor (ID): Institut Pertanian Bogor.
Jiangseubchatveera N, Liawruangrath S, Teerawutgulrag A, Santiarworn D, Pyne SG, Liawruangrath B. 2017. Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff. Chiang Mai J SCI. 44:193-202.
Mardiana E. 2017. Uji kinerja analitik spektrofotometer sinar tampak berbasis reflektans menggunakan pewarna sudan. [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Morris AS. 2001. Measurement and Instrumentation Principles. London (GB):
Butterworth-Heinemann.
Rahma N. 2016. Spektrofotometer reflektans sinar tampak dan kemometrika sebagai kendali mutu daun jati belanda (Guazuma ulmifokia). [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Sanda Fm, Victor Me, Monica Ta, Alina C. 2012. Spectrophotometric Measurements Techniques for Fermentation Process. Romania: University Of Oradea
Singh SK, Jha SK, Chaudhary, Yadava RDS, Rai SB. 2010. Quality control of herbal medicines by using spectroscopic techniques and multivariate statistical analysis. Phram Biol. 48:134-141
Stoenoiu CM, Bolbocoa AD, Jansthi L. 2006. Mobile phase optimization for steroid separation. Med Informatics. 16:141-148
Wakabayashi F, Hamada K. 2006. A DVD spectroscope: a simple, high-resolution classroom spectrosope. J Chem Educ. 83:56-58
Wiart C. 2006. Medicinal Plants of the Asia-Pacific: Drugs for the Future. Singapura: World Scientific Publishingh Co, Ltd.
Zeaiter M, Rutledge DN. 2009. Comprehensive Chemometrics. Amsterdam (NL): Elsevier.
CAMO. 2006. The Unscrambler User Manual: The Unscrambler Methods. New
York (US): CAMO Software AS.
[Depkes RI] Departemen Kesehatan Republik Indonesia. 2010. Farmakope Herbal Indonesia Edisi I. Jakarta (ID): Depkes RI.
Antari PD. 2015. Spektrofotometer Uv-Vis reflektans dan kemometrika sebagai teknik klasifikasi spesimen jaringan kanker serviks dan normal. [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Anuradha VE, Jaleel CA, Salem MA, Gomathinayagam M, Panneerselvam R. 2010. Plant growth regulators induced changes in antioksidant potential and andrographolide content in Andrographis paniculata Wall ex Nees. Pestic Biochem Physiol. 98:312-316.
Aminah, Muflihunna A, Abidin Z. 2016. Uji aktivitas antioksidan fraksi etil asetat daun wungu (Graptophyllum pictum (Linn) Griff) dengan metode FRAP (Ferric Reducing Antioxidant Power). As-syifaa. 8(1):39-44
Astuti AI. 2015. Kuantifikasi kurkuminoid dalam temulawak menggunakan spektrofotometer reflektans berbasis CCD. [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Blum-Silva CH, Chaves VC, Schenkel EP, Coelho GC, Reginatto FH. 2015. The influence of leaf age on methylxanthines, total phenolic content, and free radical scavenging capacity of Ilex paraguariensis aqueous extracts. Rev. Bras. Farmacogn. 25:1-6
Cuadros-Rodriguez L, Perez-Castano E, Ruiz-SamblasC. 2016. Quality performance metrics in multivariate classification methods for qualitative analysis. TRAC-Trend Anal Chem. 80:612-624
Dalimartha. 1999. Ramuan Tradisional Untuk Pengobatan Hepatitis. Jakarta: Penebar Swadaya.
Gromski P, Muhammadali H, Ellis D, Xu Y, Correa E. 2015. A tutorial review: metabolomics and partial least squares-discriminant analysis-a marriage of convenience or a shotgun wedding. Anal Chim Acta. 879:10-23
Hafsah N. 2017. Validasi kualitatif model klasifikasi kualitas temu lawak menggunakan spektrofotometer reflektans Quali-Vis dan Kemometrika[skripsi]. Bogor (ID): Institut Pertanian Bogor.
Jiangseubchatveera N, Liawruangrath S, Teerawutgulrag A, Santiarworn D, Pyne SG, Liawruangrath B. 2017. Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytotoxic activities of Graptophyllum pictum (L.) Griff. Chiang Mai J SCI. 44:193-202.
Mardiana E. 2017. Uji kinerja analitik spektrofotometer sinar tampak berbasis reflektans menggunakan pewarna sudan. [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Morris AS. 2001. Measurement and Instrumentation Principles. London (GB):
Butterworth-Heinemann.
Rahma N. 2016. Spektrofotometer reflektans sinar tampak dan kemometrika sebagai kendali mutu daun jati belanda (Guazuma ulmifokia). [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Sanda Fm, Victor Me, Monica Ta, Alina C. 2012. Spectrophotometric Measurements Techniques for Fermentation Process. Romania: University Of Oradea
Singh SK, Jha SK, Chaudhary, Yadava RDS, Rai SB. 2010. Quality control of herbal medicines by using spectroscopic techniques and multivariate statistical analysis. Phram Biol. 48:134-141
Stoenoiu CM, Bolbocoa AD, Jansthi L. 2006. Mobile phase optimization for steroid separation. Med Informatics. 16:141-148
Wakabayashi F, Hamada K. 2006. A DVD spectroscope: a simple, high-resolution classroom spectrosope. J Chem Educ. 83:56-58
Wiart C. 2006. Medicinal Plants of the Asia-Pacific: Drugs for the Future. Singapura: World Scientific Publishingh Co, Ltd.
Zeaiter M, Rutledge DN. 2009. Comprehensive Chemometrics. Amsterdam (NL): Elsevier.